SIT InHaVi Kit

Quick Identification Test

for strains of Salmonella Infantis, Hadar and Virchow

Due to the introduction of amendments to the Regulations No. 2160/3003 and 200/2010 in relation to the “reduction of the prevalence of Salmonella serotypes in adult breeding flocks of Gallus gallus”issued in 2011 by the EU Commission, which place particular emphasis on detection and identification of Salmonella serovars such as: S.Infantis, S.Hadar and S.Virchow,
we prepared for you the ready-to-use SIT InHaVi Kit for serological identification of these strains.

Salmonella have three types of antigen: the somatic (O), capsular Vi (some serotypes), and flagellar (H). Because of great diversity of mentioned antigens there are now 2579 Salmonella strains. Their correct identification is essential for the proper classification of  Salmonella strains.

The SIT InHaVi Kit includes necessary sera for detailed identification of the recommended strains. It is important to recognize and serologically confirm Salmonella strains while excluding other serotypes that have similar composition of flagellar antigens (shortened scheme of Kauffmann-White-Le Minor is on the website under “Download“).

SITInHaVi Kit includes:

  • 14 bottles of sera for serological identification of isolates,
  • 1 bottle of serum to inhibit the dominant phase,
  • semi-solid medium allowing proper identification of flagellar antigens,
  • clear instruction on how to use SIT InHaVi Kit.

SIT InHaVi Kit allows:

  • quick testing and identification of Salmonella Infantis, Hadar and Virchow
  • effective inhibition of dominant phase of S. Virchow and S. Infantis isolates
  • bacterial culture reinforcing flagellar antigen expression

In addition, on our website you can find a short video showing the agglutination test step by step. Click here to watch the video.

Sera in the SIT InHaVi Kit:

Sera- 5ml Sera –  3ml Sera – 2ml
1 HM 3 O:6,7 9 H:nz15 15 H:r – for phase inhibition
2 OC 4 O:7 10 H:r
5 O:8 11 H:2
6 H:z10 12 H:5
7 H:enx 13 H:6
8 H:x 14 H:7

 

Our SIT InHaVi Kit includes everything you will need to ensure proper serotype identification of Salmonella strains.

 

 

1) Commission Regulation (EU) No 517/2011

of 25 May 2011 implementing Regulation (EC) No 2160/2003 of the European Parliament and of the Council as regards a Union target for the reduction of the prevalence of certain Salmonella serotypes in laying hens of Gallus gallus and amending Regulation (EC) No 2160/2003 and Commission Regulation (EU) No 200/2010.

(Text with EEA relevance )

Article 4

Amendment to Regulation (EU) No 200/2010

In Article 1(1), the first subparagraph is replaced by the following:

‘1.   From 1 January 2010, the Union target, as referred to in Article 4(1) of Regulation (EC) No 2160/2003, for the reduction of Salmonella spp. in breeding flocks of Gallus gallus (“the Union target”) shall be a reduction to 1 % or less of the maximum percentage of adult breeding flocks of Gallus gallus remaining positive for Salmonella Enteritidis, Salmonella Infantis, Salmonella Hadar, Salmonella Typhimurium, including monophasic Salmonella Typhimurium with the antigenic formula 1,4,[5],12:i:-, and Salmonella Virchow (the relevant Salmonella serotypes).’

26.5.2011 Official Journal of the European Union L 138/45