SIT EnTy Kit

Quick Identification Test

for strains of Salmonella Enteritidis and Typhimurium

Due to the introduction of amendments to the Regulations No. 2160/3003 and 2073/2005 in relation to “Salmonella in fresh poultry meat,”1 issued in 2011 by the EU Commission, which place particular emphasis on detection and identification of Salmonella Enteritidis and Salmonella Typhimurium,
we prepared for you the ready-to-use SIT EnTy Kit for serological identification of these strains.

Salmonella have three types of antigen: the somatic (O), capsular Vi (some serotypes), and flagellar (H). Because of great diversity of mentioned antigens there are now 2579 Salmonella strains. Their correct identification is essential for the proper classification of  Salmonella strains.

The SIT EnTy  Kit includes necessary sera for detailed identification of the recommended strains. It is important to recognize and serologically confirm Salmonella strains while excluding other serotypes that have similar composition of flagellar antigens (shortened scheme of Kauffmann-White-Le Minor is on our website under “Download“).

SIT  EnTy Kit includes:

  • 13 bottles of sera for serological identification of isolates
  • 2 bottles of sera to inhibit the dominant phase
  • semi-solid medium allowing proper identification of flagellar antigens
  • clear instruction on how to use SIT EnTy Kit

SIT  EnTy Kit allows:

  • quick testing and identification of Salmonella Enteritidis and Salmonella Typhimurium
  • effective inhibition of dominant phase of S. Typhimurium
  • bacterial culture reinforcing flagellar antigen expression

In addition, on our website you can find a short video showing the agglutination test step by step. Click here to watch the video.

Sera included in the SIT EnTy Kit:

S. Enteritidis S. Typhimurium
1. HM 5 H:m 9. O:4 13. H:6
2. O:9 6 H:q 10. H:i 14. H:i for phase inhibtion
3. O:46 7 H:s 11. H:2 15. H:2 for phase inhibtion
4. H:g,m 8. H:t 12. H:5

Our SIT EnTy Kit includes everything you will need to ensure proper serotype identification of Salmonella Enteritidis and Salmonella Typhimurium.

1) Commission Regulation (EU) No 1086/2011

of 27 October 2011 amending Annex II to Regulation (EC) No 2160/2003 of the European Parliament and of the Council and Annex I to Commission Regulation (EC) No 2073/2005 as regards Salmonella in fresh poultry meat

(Text with EEA relevance)

(9) According to the Community Summary Report on trends and sources of zoonoses, and zoonotic agents and food-borne outbreaks in the European Union in 2008 (5) by the European Food Safety Authority approximately 80 % of human salmonellosis cases are caused by Salmonella Enteritidis and Salmonella Typhimurium which is similar to preceding years. Poultry meat remains a major source of  human salmonellosis.

(13) Monophasic strains of Salmonella Typhimurium have rapidly emerged as one of the most commonly found serotype of Salmonella in several species of animals and in clinical isolates from humans. According to the Scientific Opinion on monitoring and assessment of  the public health risk of ‘Salmonella Typhimurium -like’ strains (6) monophasic Salmonella Typhimurium strains with the antigenic formula 1,4,[5],12:i:- are considered as variants of Salmonella Typhimurium and current  evidence has shown that these strains appear to pose a public health risk comparable to that of other Salmonella Typhimurium strains. Therefore, it is appropriate to clarify that provisions for  Salmonella Typhimurium are applicable also to these monophasic strains.